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F-12 Nutrient Mixture (Ham) powder
[日期:2009-11-21]  来源:  作者: 
 
Contains L-glutamine but no sodium bicarbonate.
F-12 Nutrient Mixture was originally formulated for single cell plating of near-diploid Chinese Hamster Ovary (CHO) cells.

COMPONENTS

Molecular Weight

Concentration (mg/L)

Molarity (mM)

Amino Acids

Glycine

75

7.5

0.1000

L-Alanine

89

8.9

0.1000

L-Arginine hydrochloride

211

211

1.000

L-Asparagine-H2O

150

15.01

0.100

L-Aspartic acid

133

13.3

0.1000

L-Cysteine hydrochloride-H2O

176

35.12

0.200

L-Glutamic Acid

147

14.7

0.1000

L-Glutamine

146

146

1.000

L-Histidine hydrochloride-H2O

210

21

0.1000

L-Isoleucine

131

4

0.0305

L-Leucine

131

13.1

0.1000

L-Lysine hydrochloride

183

36.5

0.199

L-Methionine

149

4.5

0.0302

L-Phenylalanine

165

5

0.0303

L-Proline

115

34.5

0.300

L-Serine

105

10.5

0.1000

L-Threonine

119

11.9

0.1000

L-Tryptophan

204

2.04

0.01000

L-Tyrosine disodium salt dihydrate

225

7.81

0.0347

L-Valine

117

11.7

0.1000

Vitamins

Biotin

244

0.0073

0.0000299

Choline chloride

140

14

0.1000

D-Calcium pantothenate

477

0.5

0.00105

Folic Acid

441

1.3

0.00295

i-Inositol

180

18

0.1000

Niacinamide

122

0.036

0.000295

Pyridoxine hydrochloride

206

0.06

0.000291

Riboflavin

376

0.037

0.0000984

Thiamine hydrochloride

337

0.3

0.000890

Vitamin B12

1355

1.4

0.00103

Inorganic Salts

Calcium Chloride (CaCl2) (anhyd.)

111

33.22

0.299

Cupric sulfate (CuSO4-5H2O)

250

0.0025

0.0000100

Ferric sulfate (FeSO4-7H2O)

278

0.834

0.00300

Magnesium Chloride (anhydrous)

95

57.22

0.602

Potassium Chloride (KCl)

75

223.6

2.98

Sodium Bicarbonate (NaHCO3)

84

1176

14.00

Sodium Chloride (NaCl)

58

7599

131.02

Sodium Phosphate dibasic (Na2HPO4) anhydrous

142

142

1.000

Zinc sulfate (ZnSO4-7H2O)

288

0.863

0.00300

Other Components

D-Glucose (Dextrose)

180

1802

10.01

Hypoxanthine Na

159

4.77

0.0300

Linoleic Acid

280

0.084

0.000300

Lipoic Acid

206

0.21

0.00102

Phenol Red

376.4

1.2

0.00319

Putrescine 2HCl

161

0.161

0.001000

Sodium Pyruvate

110

110

1.000

Thymidine

242

0.7

0.00289

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