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Dulbecco's Modified Eagle Medium (D-MEM) powder (high glucose)
[日期:2009-11-21]  来源:  作者: 
 
Contains 4500 mg/L D-glucose, L-glutamine and 110 mg/L sodium pyruvate but no sodium bicarbonate.
Dulbecco's Modified Eagle Media are well suited for supporting the growth of a broad spectrum of mammalian cell lines.
Catalog Number: 12800017 , 12800082

COMPONENTS

Molecular Weight

Concentration (mg/L)

Molarity (mM)

Amino Acids

Glycine

75

30

0.400

L-Arginine hydrochloride

211

84

0.398

L-Cystine 2HCl

313

63

0.201

L-Glutamine

146

584

4.00

L-Histidine hydrochloride-H2O

210

42

0.200

L-Isoleucine

131

105

0.802

L-Leucine

131

105

0.802

L-Lysine hydrochloride

183

146

0.798

L-Methionine

149

30

0.201

L-Phenylalanine

165

66

0.400

L-Serine

105

42

0.400

L-Threonine

119

95

0.798

L-Tryptophan

204

16

0.0784

L-Tyrosine disodium salt dihydrate

261

104

0.398

L-Valine

117

94

0.803

Vitamins

Choline chloride

140

4

0.0286

D-Calcium pantothenate

477

4

0.00839

Folic Acid

441

4

0.00907

i-Inositol

180

7.2

0.0400

Niacinamide

122

4

0.0328

Pyridoxine hydrochloride

204

4

0.0196

Riboflavin

376

0.4

0.00106

Thiamine hydrochloride

337

4

0.0119

Inorganic Salts

Calcium Chloride (CaCl2) (anhyd.)

111

200

1.80

Ferric Nitrate (Fe(NO3)3"9H2O)

404

0.1

0.000248

Magnesium Sulfate (MgSO4) (anhyd.)

120

97.67

0.814

Potassium Chloride (KCl)

75

400

5.33

Sodium Chloride (NaCl)

58

6400

110.34

Sodium Phosphate monobasic (NaH2PO4-H2O)

138

125

0.906

Other Components

D-Glucose (Dextrose)

180

4500

25.00

Phenol Red

376.4

15

0.0399

Sodium Pyruvate

110

110

1.000

*Note: Pyridoxine HCl replaces pyridoxal HCl.

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