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RPMI Medium 1640
[日期:2009-11-21]  来源:  作者: 
 

RPMI Medium 1640 powder
Contains L-glutamine, but no sodium bicarbonate.
RPMI (Roswell Park Memorial Institute) Media 1640 are enriched formulations with extensive applications for mammalian cells. They were originally formulated for suspension cultures or monolayer cultures of human leukemia cells.
COMPONENTS

Molecular Weight

Concentration (mg/L)

Molarity (mM)

Amino Acids

Glycine

75

10

0.133

L-Arginine

174

200

1.15

L-Asparagine

132

50

0.379

L-Aspartic acid

133

20

0.150

L-Cystine 2HCl

313

65

0.208

L-Glutamic Acid

147

20

0.136

L-Glutamine

146

300

2.05

L-Histidine

155

15

0.0968

L-Hydroxyproline

131

20

0.153

L-Isoleucine

131

50

0.382

L-Leucine

131

50

0.382

L-Lysine hydrochloride

146

40

0.274

L-Methionine

149

15

0.101

L-Phenylalanine

165

15

0.0909

L-Proline

115

20

0.174

L-Serine

105

30

0.286

L-Threonine

119

20

0.168

L-Tryptophan

204

5

0.0245

L-Tyrosine disodium salt dihydrate

261

29

0.111

L-Valine

117

20

0.171

Vitamins

Biotin

244

0.2

0.000820

Choline chloride

140

3

0.0214

D-Calcium pantothenate

477

0.25

0.000524

Folic Acid

441

1

0.00227

i-Inositol

180

35

0.194

Niacinamide

122

1

0.00820

Para-Aminobenzoic Acid

137

1

0.00730

Pyridoxine hydrochloride

206

1

0.00485

Riboflavin

376

0.2

0.000532

Thiamine hydrochloride

337

1

0.00297

Vitamin B12

1355

0.005

0.0000037

Inorganic Salts

Calcium nitrate (Ca(NO3)2 4H2O)

236

100

0.424

Magnesium Sulfate (MgSO4) (anhyd.)

120

48.84

0.407

Potassium Chloride (KCl)

75

400

5.33

Sodium Chloride (NaCl)

58

6000

103.45

Sodium Phosphate dibasic (Na2HPO4) anhydrous

142

800

5.63

Other Components

D-Glucose (Dextrose)

180

2000

11.11

Glutathione (reduced)

307

1

0.00326

Phenol Red

376.4

5

0.013

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